The methyl green stainability of nudeic acids
نویسندگان
چکیده
منابع مشابه
Methyl Green
1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is o...
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Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content, indicate that the compound consists of one dye molecule per 10 P. The significance of this result was discussed in the preceding paper (1). Histone and lanthanum (and probably other multivalent cations (3)) compete with the dye f...
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Of all substances which bulk large in the chemical composition of cellular structures, desoxypentose nucleic acid is perhaps the most clearly demonstrable by application of cytochemical methods to cells. For each one of the three components of nucleotide residues there is a specific method which -enables the cytologist to visualize it on slides: the purines and pyrimidines have natural specific...
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Solid acids and especially those based on micelle-templated silicas and other mesoporous high surface area support materials are beginning to play a significant role in the greening of fine and specialty chemicals manufacturing processes. A wide range of important organic reactions can be efficiently catalyzed by these materials, which can be designed to provide different types of acidity as we...
متن کاملOn the Nature of the Deoxyribonucleic Acid-Methyl Green Reaction
Methyl green has frequently been used for histochemical and in vitro demonstration of deoxyribonucleic acid (DNA). I t has been reported that this color or staining reaction requires the presence of highly polymeric DNA (1) and that its molecular size influences the extent of the reaction. Thus, as the DNA is broken down by deoxyribonuclease (DNase), there is a corresponding decrease in the bin...
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ژورنال
عنوان ژورنال: The Journal of the Japanese Society of Clinical Cytology
سال: 1978
ISSN: 1882-7233,0387-1193
DOI: 10.5795/jjscc.17.64